The current group works on multiple techniques for spatial omics mapping. We are continuously expanding our toolsets with new designs.
01 DBiT-seq
Deterministic Barcoding in Tissue for spatial omics sequencing – for co-mapping of mRNAs and proteins in a formaldehyde-fixed tissue slide via NGS sequencing. Parallel microfluidic channels were used to deliver DNA barcodes to the surface of a tissue slide and crossflow of two sets of barcodes A1-50 and B1-50 followed by ligation in situ yielded a 2D mosaic of tissue pixels, each containing a unique full barcode AB. Read more.
02 DBiT-seq FFPE
Formalin-fixed paraffin-embedded (FFPE) tissues are the most abundant archivable specimens in clinical tissue banks, but unfortunately incompatible with single-cell level transcriptome sequencing due to RNA degradation in storage and RNA damage in extraction. We developed an in-tissue barcoding approach namely DBiT-seq for spatially resolved whole transcriptome sequencing at the cellular level, which required no tissue dissociation or RNA exaction, thus potentially more suited for FFPE samples. Read more.
03 Spatial CITE-seq
Spatial-CITE-seq for high-plex protein and whole transcriptome co-mapping in tissue can map 189 proteins and whole transcriptome in multiple mouse tissue types. It was also able to map human tissues with up to 273 proteins and transcriptome. Read more.